mouse monoclonal anti timp2  (R&D Systems)

Journal: International Journal of Oral Science

Article Title: Transcriptome analysis of ankylosed primary molars with infraocclusion

doi: 10.1038/s41368-019-0070-1

Figure Lengend Snippet: Summary of immunostained proteins in the P1 molar tooth germ for the six genes under consideration

Article Snippet: Primary antibodies were: rabbit polyclonal anti-periostin (POSTN) (1:100, LifeSpan Biosciences, Seattle), mouse monoclonal anti-tissue inhibitor of metalloproteinases 2 (TIMP2) (1:400, Millipore, Temecula), rabbit polyclonal anti-matrix metalloproteinase 2 (MMP2) (1:50, Millipore, Temecula), mouse monoclonal anti-cytokeratin 14 (CK14) (1:200, Thermo Fisher Scientific, Rockford), mouse monoclonal anti-desmoglein 3 (DSG3) (1:100, Thermo Fisher Scientific, Rockford) and mouse monoclonal anti-plakophilin 1 (PKP1) (1:50, Abcam, Cambridge).

Techniques: Expressing

Journal: International Journal of Oral Science

Article Title: Transcriptome analysis of ankylosed primary molars with infraocclusion

doi: 10.1038/s41368-019-0070-1

Figure Lengend Snippet: Immunohistochemical staining of proteins encoded by over-expressed (CK14, DSG3, PKP1) and under-expressed (POSTN, TIMP2, MMP2) genes in infraocclusion. a Hematoxylin and Eosin staining of P1 mouse molar tooth germ in the sagittal plane with various nascent structures within the dental follicle. b CK14 was strongly stained in the dental follicle. c Higher magnification of boxed area showed CK14 immunostaining in the stellate reticulum, stratum intermedium, inner enamel epithelium and ameloblast layer. d DSG3 was strongly stained in the dental follicle and to a lesser extent in the ameloblast layer. e PKP1 was strongly stained in the dental follicle, as well as the inner enamel epithelium and the apical aspect of the ameloblast layer. f POSTN was immunostained only in the dental follicle. g MMP2 was expressed only in the dental follicle and stellate reticulum. h TIMP2 was the only protein in this sample not expressed in the dental follicle, but was abundantly present in the ameloblast and odontoblast layers. Scale bars are 100 μm

Article Snippet: Primary antibodies were: rabbit polyclonal anti-periostin (POSTN) (1:100, LifeSpan Biosciences, Seattle), mouse monoclonal anti-tissue inhibitor of metalloproteinases 2 (TIMP2) (1:400, Millipore, Temecula), rabbit polyclonal anti-matrix metalloproteinase 2 (MMP2) (1:50, Millipore, Temecula), mouse monoclonal anti-cytokeratin 14 (CK14) (1:200, Thermo Fisher Scientific, Rockford), mouse monoclonal anti-desmoglein 3 (DSG3) (1:100, Thermo Fisher Scientific, Rockford) and mouse monoclonal anti-plakophilin 1 (PKP1) (1:50, Abcam, Cambridge).

Techniques: Immunohistochemical staining, Staining, Immunostaining

Journal: Cancer cell

Article Title: CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes

doi: 10.1016/j.ccell.2017.04.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal anti-TIMP2 , Thermo Fisher , Cat#MA1-774; RRID: AB_889483.

Techniques: Recombinant, Transfection, Protease Inhibitor, SYBR Green Assay, Reverse Transcription, Isolation, DNA Methylation Assay, DNA Purification, Chromatin Immunoprecipitation, CRISPR, Methylation, Software

Journal: European Journal of Histochemistry : EJH

Article Title: The anti-inflammatory effects of exercise training promote atherosclerotic plaque stabilization in apolipoprotein E knockout mice with diabetic atherosclerosis

doi: 10.4081/ejh.2013.e3

Figure Lengend Snippet: Effects of exercise training on IL-6, MMP-2, MMP-3, MMP-8 and TIMP-2 content of the atherosclerotic lesions. CO, control group; SED, sedentary group; EX, exercise group. Results are expressed as mean ± standard deviation.*P<0.05, EX compared to CO group; #P<0.05, EX compared to SED group.

Article Snippet: Consecutive sections were also stained with polyclonal antibodies against MMP-2 and MMP-3 (MBL International Corporation, Woburn, MA, USA), MMP-8 (Chemicon International Inc., Temecula, CA, USA), TIMP-2 (Acris Antibodies GmbH, Herford, Germany) or rat monoclonal anti-mouse IL-6 (Biolegends, San Diego, CA, USA).

Techniques: Standard Deviation